Castelli et al. Crit Care (2021) 25:137 https://doi.org/10.1186/s13054-021-03572-y RESEARCH LETTER Open Access Cerebral venous sinus thrombosis associated with thrombocytopenia post-vaccination for COVID-19 Gian Paolo Castelli1* , Claudio Pognani1, Carlo Sozzi2, Massimo Franchini3 and Luigi Vivona4 Introduction Cerebral venous sinus thrombosis (CVST) is a rare form of stroke generally occurring in younger patients (typi- cally < 50 years old), predominantly women and amount- ing to 0.5–1% of all strokes. Incidence is about 5–16 cases per 1 million people per year [1–3]. CVST has been reported in COVID-19 patients asso- ciated with thrombocytopenia [4, 5]; on March 15, 2021, the Paul Ehrlich Institut (Federal Institute for vaccines and biomedicines) reported CVST in seven patients 20–50  years old after vaccination with COVID-19 vac- cine AstraZeneca. We report about a previously healthy 50-year-old Cau- casian man admitted to the city hospital of Mantua on March 15, 2021, with severe headache during the pre- vious four days, slight deviation of the right buccal rim, loss of strength in the right lower limb, unstable walking and slight visual impairment. On March 4, 2021, he had received the first dose of the anti-COVID-19 AstraZen- eca vaccine with no immediate adverse reaction. On examination, he was apyretic, arterial pressure 150/80 mmHg and heart rate 80/min, SpO2 99% in room air, GCS 15, pain numerical rating scale 8/10. Labora- tory blood tests showed marked abnormalities in blood coagulation (Table 1). The patient was a volunteer blood donor, and previous routine blood tests had repeatedly reported normal platelet counts. SARS-CoV-2 Buffer *Correspondence: gianpaolo.castelli@gmail.com 1 Department of Anesthesiology and Intensive Care, “Carlo Poma” Hospital, ASST Mantova, Via Lago Paiolo, 10, 46100 Mantova, Italy Full list of author information is available at the end of the article (RT-PCR) and Anti-SARS-CoV-2 Antibody Search were negative. A brain CT scan showed intra-parenchymal haemorrhage in the left hemisphere, while CT angiog- raphy showed multiple bleeding spots within the paren- chymal haemorrhage and lack of opacification of the left transverse and sigmoid sinuses, suggesting thrombosis of the venous sinuses (Fig.  1). Four hours after admis- sion, the patient had deteriorated to GCS 8, right hemi- plegia, localization of the painful stimulus to the left, no execution of orders nor verbal production. He showed isochoric, isocyclic pupils and vomiting. The patient was transferred to the intensive care unit (ICU), and a throm- boelastogram (TEG6S, Haemonetics) showed a pro- longed reaction time, a decreased platelet function and lack of fibrinogen, with marked reduction of maximum amplitude of the clot; fibrinogen concentrate (10 g total) and platelet (4 units total) were administered. Six hours after ICU admission, the patient had become medium mydriatic: he was intubated, sedated and para- lysed and underwent a second CT scan, which docu- mented the increase in the haemorrhagic focus, the initial transtentorial herniation of the left temporal uncus and a shift of the midline to the right. The patient underwent a bilateral decompressive craniectomy, which confirmed diffuse thrombosis of the cortical veins. Upon returning from the operating room, a subsequent TEG6S showed normalization of the clot formation rate and reduced clot strength, with PLT had fall back to 15,000/mcL. Antiplatelet antibodies were negative, while a heterozy- gous state for the MTHFR thrombophilic mutation with increased levels of hyperhomocysteine and concomitant folate deficiency were reported post-mortem. © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. The Creative Commons Public Domain Dedication waiver (http:// creat iveco mmons. org/ publi cdoma in/ zero/1. 0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Castelli et al. Crit Care (2021) 25:137 Page 2 of 2 Table 1 Abnormal laboratory parameters Parameter Patient’s values Normal values the data; GPC, MF and LV contributed in data analysis, interpretation of the data and drafting the manuscript. All authors read and approved the final manuscript. Platelets (109/L) Fibrinogen (mg/dL) D-dimer (ng/mL) C reactive protein (mg/L) Coagulation factor XIII (%) Homocysteine (μmol/L) Folic acid (ng/mL) > 10,000 20 98 17.6 35 16.7 0.9 150–400 150–450 < 500 < 5 70–150 Absent < 12 3.9–26.8 Methylenetetrahydrofolate reduc- tase (MTHFR) mutation (C677T) Heterozygous Declarations Funding No funds have been received and will be received for this study. Availability of data and materials The dataset used and/or analysed during the current study is available from the corresponding author on reasonable request. Ethics approval and consent to participate The need for informed consent from individual patients was waived owing to the retrospective and observational nature of the study. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests. Author details 1 Department of Anesthesiology and Intensive Care, “Carlo Poma” Hospital, ASST Mantova, Via Lago Paiolo, 10, 46100 Mantova, Italy. 2 Neuroradiology, “Carlo Poma” Hospital, ASST Mantova, Mantova, Italy. 3 Department of Hema- tology and Transfusion Medicine, “Carlo Poma” Hospital, ASST Mantova, Man- tova, Italy. 4 Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy. Received: 6 April 2021 Accepted: 8 April 2021 Fig.1 3D reconstruction with lack of opacification of the left transverse sinus Haemodynamic instability, mydriasis and lack of intra- cerebral blood flow at CT angiography led to the diagno- sis of brain death, approximately 48 h after admission to the hospital. 2. 3. Discussion SARS-CoV-2 infection has been associated with hyper- coagulability, with a high incidence of venous thrombo- embolism including pulmonary embolism and deep vein thrombosis. Physicians should also be alert for signs and symptoms related to thromboembolism when they occur in patients who have recently been vaccinated with the COVID-19 AstraZeneca vaccine. Acknowledgements Not applicable. Authors’ contributions GPC, CP, CS and MF conceived and designed the study; GPC, CS and MF contributed in data management; GPC, CP, CS and MF collected and analysed References 1. Saposnik G, Barinagarrementeria F, Brown RD Jr, American Heart Associa- tion Stroke Council and the Council on Epidemiology and Prevention, et al. Diagnosis and management of cerebral venous thrombosis: a state- ment for healthcare professionals from the American Heart Association/ American Stroke Association. Stroke. 2011;42(4):1158–92. https:// doi. org/ 10. 1161/ STR. 0b013 e3182 0a8364. Ferro JM, Bousser MG, Canhão P, et al. European Stroke Organization guideline for the diagnosis and treatment of cerebral venous throm- bosis—endorsed by the European Academy of Neurology. 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